Preparation and Reinfusion of Autologous Protein Isolated from Maligant Pleural Effusion and Ascites 恶性胸腹水自体蛋白的制备及回输
Methods SERPA of four human lung adenocarcinoma tissues were performed. The Western blot imaging films which reacted with autologous patient serum and with control normal serum were obtained and the differential reacting protein spots were recognized. 方法应用SERPA对4例人肺腺癌组织进行研究,分析肺腺癌组织总蛋白分别与肺腺癌患者的自身血清以及正常对照血清反应的westernblot图谱,识别鉴定差异反应的蛋白质。
Objective To study the effect of autologous hematopoietic stem cell ( HSC) mobilized by granulocyte colony stimulating factor on the expression of bcl-2 and bax protein in focal cerebral ischemia/ reperfusion middle cerebral artery occlusion ( MCAO/ R) rats. 目的探讨粒细胞集落刺激因子(G-CSF)动员自体造血干细胞对大脑中动脉栓塞/再灌注(MCAO/R)大鼠细胞凋亡相关基因bcl-2、bax表达的影响。